NOVELTY – The method involves diluting immunoglobulin with a buffer, and injecting diluted immunoglobulin into a chromatography column, where the chromatography column contains stationary phase polyamine-agarose. High ionic strength protein is adsorbed in a stationary phase. The chromatography column is regenerated with sodium hydroxide solution. USE – Method for purifying immunoglobulin G (IgG) from human or animal serum by gel chromatography. ADVANTAGE – The method avoids bacterial contamination during purification process, and ensures high degree of pureness to the immunoglobulin in a cost-effective manner.

Main Application Field

A88 (Mechanical engineering and tools e.g. valves, gears and conveyor belts.); B04 (Natural products and polymers. Including testing of body fluids (other than blood typing or cell counting), pharmaceuticals or veterinary compounds of unknown structure, testing of microorganisms for pathogenicity, testing of chemicals for mutagenicity or human toxicity and fermentative production of DNA or RNA. General compositions.); D16 (Fermentation industry – including fermentation equipment, brewing, yeast production, production of pharmaceuticals and other chemicals by fermentation, microbiology, production of vaccines and antibodies, cell and tissue culture and genetic engineering.); J01 (Separation – including evaporation, crystallisation, solvent extraction, chromatography, dialysis, osmosis including drying gases and/or vapours, and separation of solids from gases, liquids and other solids. Isotope separation, filter materials (including molecular sieves for separation), and centrifuges (except where used for analysis) (B01D, B03, B04, B07B).); J04 (Chemical/physical processes/apparatus – including catalysis, catalysts (excluding specific e.g. enzymatic or polymerisation catalysts), colloid chemistry, laboratory apparatus and methods, testing, controlling, general encapsulation, detection and sampling (excluding clinical testing) (B01J, L).); S03 (Scientific Instrumentation)

INVENTORS:

MARTINS DE SOUZA MARIA CRISTIANE
LAZZAROTTO BRESOLIN IGOR TADEU
ANTUNES PEREIRA IARA ROCHA
ALVES MIRANDA EVERSON
ALVES BUENO SONIA MARIA

333_SORO

Patent number: BR200900276-A2

PATENT STATUS:

For information contact Inova Unicamp

FOR ADDITIONAL INFORMATION:

parcerias@inova.unicamp.br

+55 (19) 3521-5207 / 2607

This technology profile has been automatically generated.