NOVELTY – Lignocellulolytic enzyme composition comprises superoxide dismutase having amino acids (SEQ ID NO: 1) and cellulases. USE – Lignocellulolytic enzyme composition used for recovery and conversion of lignocellulosic materials for the production of second generation ethanol or other high added by-products. ADVANTAGE – Lignocellulolytic enzyme composition allows greater release of monomeric sugars for the production of second generation ethanol or other value added by-products. DETAILED DESCRIPTION – INDEPENDENT CLAIMS are included for: (1) a method for performing enzymatic conversion, which involves pretreating lignocellulosic material; (2) incubating the pre-treated lignocellulosic material with the composition; (3) a vector, which comprises replication origin sequence, selection marker sequence and multiple cloning sites which comprises (SEQ ID NO: 2) operably linked to the heterologous promoter and terminator sequences; and (4) an expression vector, which comprises T7 promoter (370-386), T7 transcription primer (369), coding sequence for His tag (270-287), coding sequence for T7 Tag (207 – 239), multiple cloning sites, including the NheI and BamHI sites, coding sequence for His.tag (140-157), terminator T7 (26-72), coding sequence for lacI (773-1852), source PBR322 origin (3286), coding sequence Kan (kanamicin) (3995-4807) and f1 origin (4903-5358) which comprises (SEQ ID NO: 2) inserted between the Nhel and SamHI sites. (Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication).

Main Application Field

D16 (Fermentation industry – including fermentation equipment, brewing, yeast production, production of pharmaceuticals and other chemicals by fermentation, microbiology, production of vaccines and antibodies, cell and tissue culture and genetic engineering.); E17 (Other aliphatics.)

INVENTORS:

PEREIRA GONÇALO AMARANTE GUIMARÃES
CAIRO JOÃO PAULO LOURENÇO
SQUINA FÁBIO MARCIO
CARAZZOLE MARCELO FALSARELLA
MANDELLI FERNANDA
PRADELLA JOSÉ GERALDO DA CRUZ
ALVARES THABATA MARIA
LEONARDO FLÁVIA COSTA
COSTA FERNANDO FERREIRA
LEONARDO ANA MARIA COSTA
TRAMONTINA ROBSON
DE PAIVA LIVIA BEATRIZ BRENELLI
GONÇALVES THIAGO AUGUSTO
ROBL DIOGO

955_CUPIM

Patent number: WO2017011885-A1;BR102015017256-A2

PATENT STATUS:

For information contact Inova Unicamp

FOR ADDITIONAL INFORMATION:

parcerias@inova.unicamp.br

+55 (19) 3521-5207 / 2607

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